mouse immortalized leydig cell lines  (ATCC)

Journal: Current Research in Toxicology

Article Title: Ethylene dimethanesulfonate effects on gene promoter activities related to the endocrine function of immortalized Leydig cell lines R2C and MA-10

doi: 10.1016/j.crtox.2023.100147

Figure Lengend Snippet: (A) Representative aspect of cells after 24 h of exposure to ethylene dimethanesulfonate (EDS) of rat R2C and mouse MA-10 Leydig cells. Note that at 5 mM, the cell confluency and morphological characteristic seem qualitatively impaired (red arrows; insert), indicating the cytotoxicity caused by EDS. (B) Cell count of R2C and MA-10 cells after 4 and 24 h of EDS exposure at concentrations of 1 or 2 mM (n = 4, each in duplicate). Values are expressed as median (interquartile ranges). Kruskal-Wallis followed by Dunn’s test (p > 0.05).

Article Snippet: Rat and mouse immortalized Leydig cell lines, R2C (ATCC, Manassas, Virginia, USA) and MA-10 (ATCC, Manassas, Virginia, USA), respectively, were used for this study from passages 40 to 52 (R2C) or 08 to 31 (MA-10).

Techniques: Cell Counting

Journal: Current Research in Toxicology

Article Title: Ethylene dimethanesulfonate effects on gene promoter activities related to the endocrine function of immortalized Leydig cell lines R2C and MA-10

doi: 10.1016/j.crtox.2023.100147

Figure Lengend Snippet: A dose–response study with reporter luciferase assays to determine the effects of EDS on (A) Star , (B) Cyp17a1 , (C) Insl3 , and (D) Gsta3 promoter activity. Rat R2C (n = 4, each in triplicate) and mouse MA-10 (n = 5, each in triplicate) Leydig cells were transfected with different promoter constructs and treated for 24 h with increasing concentrations of EDS as indicated. Luciferase reporter (Luc). Values are expressed as mean ± S.E.M. ANOVA followed by Dunnett’s test (*p < 0.05 compared to the control group).

Article Snippet: Rat and mouse immortalized Leydig cell lines, R2C (ATCC, Manassas, Virginia, USA) and MA-10 (ATCC, Manassas, Virginia, USA), respectively, were used for this study from passages 40 to 52 (R2C) or 08 to 31 (MA-10).

Techniques: Luciferase, Activity Assay, Transfection, Construct, Control

Journal: Current Research in Toxicology

Article Title: Ethylene dimethanesulfonate effects on gene promoter activities related to the endocrine function of immortalized Leydig cell lines R2C and MA-10

doi: 10.1016/j.crtox.2023.100147

Figure Lengend Snippet: EDS effects on the activity of the Insl3 and Gsta3 gene promoter after 4 and 24 h of exposure to EDS of rat R2C (1 mM of EDS, n = 4–5, each in triplicate) and mouse MA-10 (2 mM of EDS, n = 5, each in triplicate) Leydig cells. Luciferase reporter (Luc). Values are expressed as mean ± S.E.M. Welch’s t -test (*p < 0.05).

Article Snippet: Rat and mouse immortalized Leydig cell lines, R2C (ATCC, Manassas, Virginia, USA) and MA-10 (ATCC, Manassas, Virginia, USA), respectively, were used for this study from passages 40 to 52 (R2C) or 08 to 31 (MA-10).

Techniques: Activity Assay, Luciferase

Journal: Current Research in Toxicology

Article Title: Ethylene dimethanesulfonate effects on gene promoter activities related to the endocrine function of immortalized Leydig cell lines R2C and MA-10

doi: 10.1016/j.crtox.2023.100147

Figure Lengend Snippet: EDS effects on Star promoter activity after 4 h of exposure to EDS of rat R2C (1 mM of EDS, n = 5, each in triplicate) and mouse MA-10 (2 mM of EDS, n = 5, each in triplicate) Leydig cells. EDS effects on Star promoter activity after 24 h of exposure to EDS of rat R2C (1 mM of EDS, n = 3, each in triplicate) and mouse MA-10 (2 mM of EDS, n = 5, each in triplicate) Leydig cells, DC3 granulosa cells (1 mM of EDS, n = 5, each in triplicate), and MSC-1 Sertoli cells (2 mM of EDS, n = 5, each in triplicate). EDS effects were assessed in basal and stimulated (0.5 mM 8Br-cAMP) conditions in R2C Leydig cells. Luciferase reporter (Luc). Values are expressed as mean ± S.E.M. Welch’s t -test (*p < 0.05).

Article Snippet: Rat and mouse immortalized Leydig cell lines, R2C (ATCC, Manassas, Virginia, USA) and MA-10 (ATCC, Manassas, Virginia, USA), respectively, were used for this study from passages 40 to 52 (R2C) or 08 to 31 (MA-10).

Techniques: Activity Assay, Luciferase

Journal: Current Research in Toxicology

Article Title: Ethylene dimethanesulfonate effects on gene promoter activities related to the endocrine function of immortalized Leydig cell lines R2C and MA-10

doi: 10.1016/j.crtox.2023.100147

Figure Lengend Snippet: The EDS-responsive region is located between −400 and −195 bp of the Star promoter. Progressive 5′ deletion constructs of Star gene promoter were transfected in rat R2C Leydig cells and treated with 1 mM EDS for 24 h (n = 3–5, each in triplicate) in the absence or presence of 0.5 mM 8Br-cAMP for 4 h. Luciferase reporter (Luc). Values are expressed as mean ± S.E.M. Welch’s t -test (*p < 0.05).

Article Snippet: Rat and mouse immortalized Leydig cell lines, R2C (ATCC, Manassas, Virginia, USA) and MA-10 (ATCC, Manassas, Virginia, USA), respectively, were used for this study from passages 40 to 52 (R2C) or 08 to 31 (MA-10).

Techniques: Construct, Transfection, Luciferase